What is the most common animal model used for Type II diabetes in humans?

Other diet-induced rodent models of type 2 diabetes Although rats and mice are the most commonly used models for studies of type 2 diabetes, other rodents have also been identified as useful models.

Do mice get type 2 diabetes?

These data suggest that the C57BL/6J mouse carries a genetic predisposition to develop non-insulin-dependent (type II) diabetes. Futhermore, altered glycemic response to adrenergic stimulation may be a biologic marker for this genetic predisposition to develop type II diabetes.

How do you induce type 2 diabetes in mice?

To induce diabetes, animals of both sexes received daily intraperitoneal (i.p.) injection of STZ for 5 consecutive days at 55 mg/kg BW (a dose that is known to induce diabetes in male mice) or for females, 75 mg/kg BW of STZ.

Why is type 2 diabetes so bad?

Type 2 diabetes is an impairment in the way the body regulates and uses sugar (glucose) as a fuel. This long-term (chronic) condition results in too much sugar circulating in the bloodstream. Eventually, high blood sugar levels can lead to disorders of the circulatory, nervous and immune systems.

When is a mouse diabetic?

Non-obese diabetic (NOD) mice are a well recognized model of spontaneous type 1 diabetes and have a long prodrome of pre-diabetes from 6–8 weeks of age prior, progressing to spontaneous diabetes at approximately 18–24 weeks of age, the stage where islet destruction sufficient for hyperglycemia occurs.

How do mice cause diabetes?

Diabetes is induced in mice by using streptozotocin (STZ), a compound that has a preferential toxicity toward pancreatic β cells.

How do they test for diabetes in mice?

Biochemical assays, such as glucose oxidase assays, are another common way to measure blood glucose from your mice. There are many such colorimetric or fluorometric assays that are coupled with enzymes to cause a colormetric change that can be measured spectroscopically.

How do you test for diabetes in mice?

Insulin tolerance tests The convention is to conduct ITTs in mice following a short (5- to 6-hour) fast. Glucose concentration is monitored every 15 to 30 minutes for 60 to 90 minutes following a bolus of insulin administered via intraperitoneal or intravenous injection.

When do NOD mice develop diabetes?

Based on data from the NOD/ShiLtJ strain, NOD mice maintained under SPF conditions develop insulitis between 2 and 4 weeks in females and within 5 to 7 weeks in males. Diabetes develops in 90\% to 100\% of females by 30 weeks; in contrast, only 50\% to 80\% of males.