What is the function of each one of the Gram stain reagents?

The first reagent is called the primary stain. Its function is to impart its color to all cells. The second stain is a mordant used to in- tensify the color of the primary stain. In order to es- tablish a color contrast, the third reagent used is the decolorizing agent.

How does the bacterial cell structure contribute to the mechanism of Gram staining?

How does the bacterial cell structure contribute to the mechanism of Gram staining? Gram-positive cell has a thick wall and retains crystal violet dye better. Gram-negative cell has a thin wall. Over decolorization may be a result of over decolorizing and cause your stain to be gram-staining.

What is the cell structure that allows the Gram staining process to work?

Gram staining differentiates bacteria by the chemical and physical properties of their cell walls. Gram-positive cells have a thick layer of peptidoglycan in the cell wall that retains the primary stain, crystal violet.

What is the theory about the mechanism of the Gram stain reaction?

With the current theory behind Gram staining, it is thought that in Gram-positive bacteria, the crystal violet and iodine combine to form a larger molecule that precipitates out within the cell.

What are the procedure of staining?

Procedure of Gram Staining

1. Take a clean, grease free slide.
2. Prepare the smear of suspension on the clean slide with a loopful of sample.
3. Air dry and heat fix.
4. Crystal Violet was poured and kept for about 30 seconds to 1 minutes and rinse with water.
5. Flood the gram’s iodine for 1 minute and wash with water.

What is the function of Gram staining?

Introduction: Gram staining is a method commonly used to determine the chemical make up of the cell wall of bacteria. The cell wall can stain either positive or negative, depending on its chemistry. Knowing the chemical make up makes it easier to manipulate the bacteria for various purposes.

What is the most important step in the Gram staining procedure Why?

The thickness of the smear used in the Gram stain will affect the result of the stain. The step that is most crucial in effecting the outcome of the stain is the decolorizing step.

What is the principle behind the Gram stain reaction explain briefly quizlet?

What is the current theory about the mechanism of the gram stain reaction? The gram stain is a basic differential stain used to determine if a bacterial cell is gram positive or negative. Gram positive cells have a thick peptidoglycan layer that will trap the crystal violet iodine crystals and appear purple.

What are the steps to Gram staining?

There are six basic steps:

1. Apply a smear of bacteria on to a slide.
2. Add about 5 drops of Hucker’s Crystal Violet to the culture.
3. Add about 5 drops of iodine solution to the culture.
4. Tilt slide and decolorize with solvent (acetone-alcohol solution) until purple color stops running.
5. Add about 5 drops of Safranine O.