What is Gram staining and explain its procedure?

Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet.

How do you perform a Gram stain procedure?

There are six basic steps:

1. Apply a smear of bacteria on to a slide.
2. Add about 5 drops of Hucker’s Crystal Violet to the culture.
3. Add about 5 drops of iodine solution to the culture.
4. Tilt slide and decolorize with solvent (acetone-alcohol solution) until purple color stops running.
5. Add about 5 drops of Safranine O.

What is the process of staining?

Staining – application of stain to a sample to color cells, tissues, components, or metabolic processes. This process may involve immersing the sample (before or after fixation or mounting) in a dye solution and then rinsing and observing the sample under a microscope.

What is the principle of acid-fast staining?

Principle of Acid-Fast Stain When the smear is stained with carbol fuchsin, it solubilizes the lipoidal material present in the Mycobacterial cell wall but by the application of heat, carbol fuchsin further penetrates through lipoidal wall and enters into cytoplasm. Then after all cell appears red.

On what principle is differential staining based?

The differential nature of the Gram stain is based on the ability of some bacterial cells to retain a primary stain (crystal violet) by resisting a decolorization process. Gram staining involves four steps. First cells are stained with crystal violet, followed by the addition of a setting agent for the stain (iodine).

What is the principle of acid fast staining?

What is the importance of Gram staining?

The main benefit of a gram stain is that it helps your doctor learn if you have a bacterial infection, and it determines what type of bacteria are causing it. This can help your doctor determine an effective treatment plan.

What is the principle of Gram and acid fast staining?

What is the principle of capsule staining?

A positive capsule stain requires a mordant that precipitates the capsule. By counterstaining with dyes like crystal violet or methylene blue, bacterial cell wall takes up the dye. Capsules appear colourless with stained cells against dark background.

What is simple staining procedure?

Simple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background.