What do you mean by ion exchange chromatography?

Ion chromatography (or ion-exchange chromatography) separates ions and polar molecules based on their affinity to the ion exchanger. In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it.

Why do we use ion exchange chromatography?

Ion exchange chromatography is commonly used to separate charged biological molecules such as proteins, peptides, amino acids, or nucleotides. The amino acids that make up proteins are zwitterionic compounds that contain both positively and negatively charged chemical groups.

How do you do ion exchange chromatography?

Ion-exchange chromatography is generally a four-step process. First, a packed column containing either anion- or cation-exchange resin is equilibrated using buffer. For anion-exchange columns, this involves protonating the resin, ensuring it is positively charged. Next, the sample is loaded on the column.

What is chromatography used for in chemistry?

chromatography, technique for separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream, called the mobile phase, and a contiguous stationary phase.

What are the types of ion exchange?

There are two general types of ion exchange resins: those that exchange positive ions, called cation exchange resins, and those that exchange negative ions, called anion exchange resins. A cation is an ion with a positive charge.

Is ion exchange chromatography and HPLC?

Ion chromatography is a modified version of HPLC with a capacity for precise and highly sensitive detection of inorganic ions in a complex matrix. Ion chromatography has instrumental configurations similar to those of HPLC: a pumping system, a column compartment, and a detecting system.

What is chromatography with example?

A technique used to separate the components of a chemical mixture by moving the mixture along a stationary material, such as gelatin. An example of chromatography is when a chemical reaction is used to cause each of the different size molecules in a liquid compound to separate into their own parts on a piece of paper.

What is an ion exchange system and how does it work?

Ion exchange (IX) systems are used across a variety of industries for water softening, purification, and separation purposes. While the chemistry of individual ion exchange reactions varies from one application to the next, IX is a treatment process where dissolved ions are replaced by other, more desirable, ions of a similar electrical charge.

What does ion exchange mean?

Ion exchange is an exchange of ions between two electrolytes or between an electrolyte solution and a complex. In most cases the term is used to denote the processes of purification, separation, and decontamination of aqueous and other ion-containing solutions with solid polymeric or mineralic “ion exchangers”.

What are the basics of chromatography?

The basic principle of chromatography is that different chemicals have different degrees of dissolving power in a liquid, and different powers of sticking to a solid surface. Thus, chromatography can identify chemical components in a mixture, and separate them by making them visible on a surface.

What are the uses of chromatography?

Separating Colored Pigments. Paper chromatography is an effective technique for separating colored pigments from a mixture.