How do you remove the supernatant from a 96-well plate?
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How do you remove the supernatant from a 96-well plate?
Flick (cells don’t need to be kept sterile): Spin down the round/U-bottom 96-well plate(s) in a centrifuge for ~ 1 minute at ~1000 rpm. Place one or more paper towels at the ready by the sink to tamp the plate wells.
How to remove supernatant?
The supernatant can be removed by either decanting it – a fancy name for pouring it off, or it can be aspirated – a fancy term for using suction to remove it. The purified specimen can then be returned to a solution via a process called, resuspending.
How do you aspirate supernatant?
Aspirate the supernatant simply means just throw the supernatant and work with pellet.
How do you clean suspension cells in a 96 well plate?
Wash the cells by pipetting 200 μl ice-cold PBS into each well. Remove the PBS from the bottom of the wells by gentle aspiration. Repeat two times for a total of three washings. Pipette 30 μl protease-inhibitor-supplemented Cell Extraction Buffer into each well.
How do you recover supernatant?
Try adding incremental amounts of NaCl. It should reduce viscosity and allow recovery of more supernatant.
Why is it important to only take from supernatant?
Purification of water to remove copper in the water generated by the PCB processes in the electronics industry. The supernatant in this case contains the excess corrosive copper. By eliminating this supernatant, the waste water becomes free of the corrosive and toxic properties and hence environmentally friendly.
How do you decant a supernatant solution?
How to Separate a Supernatant from a Precipitate by Decantation:
- Put a stirring stick on the mouse of the container to avoid trickling of the liquid to the outside of the container.
- Tilt the container and slowly decant the supernatant.
How do you separate supernatant and pellet?
The supernatant is the solution above the solid that has been forced to the bottom of the centrifuge tube. To remove the supernatant, carefully pour or pipette the solution away from the solid. If the solid becomes re-suspended as the supernatant is removed, centrifuge the sample again.
How do you aspirate a well?
How to aspirate: Aspirate by immersing the tips just below the liquid’s surface (2-3 mm) Touch off after dispense: Touch off after each dispense when removing the pipette from the target vessel. Pre-wet: Pre-wet by aspirating and dispensing the nominal volume 3 times.
How do you clean suspension cells?
Wash the cells by pipetting 200 μl ice-cold PBS into each well. Remove the PBS from the bottom of the wells by gentle aspiration. Repeat two times for a total of three washings.