How do you clean a peek tubing?

If the proteins are highly sticky then you can try using 1N NaOH to clean the peek tubing. Other alternatives are 2\% Contrad (detergent), 0.5\% SDS or 70\% Isopropanol. The assay temperature is around room temp or slightly above at 26C.

Can you sonicate peek?

Submerge the tubing in the solution in an appropriately sized beaker then sonicate. If you can attach a syringe to the end then try filling the syringe whilst sonicating (you may need some assistance to ensure the other end of the PEEK tubing stays in the methanol solution).

How do you get rid of contamination in HPLC?

If glassware or solvent reservoirs become contaminated with microbial growth, treat them in an autoclave. Remove and replace all filters and tubing between the mobile phase reservoir and the instrument. Finally, purge the system with acetonitrile or methanol and let it sit overnight.

How do you clean HPLC tubing?

You can try to reverse the tubing and flush with 100\% water. Start with a high flow rate (5 mL/min) for 10-15 seconds and check the pressure on your system. If the pressure of the system drops gradually, that means the front part of the tubing has clogged.

How do I unclog a column in HPLC?

First, wash the column with varying solvents at a low flow rate. However, if your column is so clogged that it can’t flow at all, flip the column and run it in the opposite direction. If all else fails, then you might need a new column. Like I said earlier, columns do have a finite life.

How do you clean HPLC glasses?

1. Clean glassware:

1. Three rinses with high purity methanol (we use Burdick & Jackson HPLC grade)
2. Three rinses with 1.2 M HCl (10\% concentrated HCl in water)
3. Three rinses with 5\% HF(aq) (10 mL 50\% HF in 100 mL Milli Q water). Wear gloves, a lab coat.

How do you clean LCMS glasses?

Cleaning Laboratory Glassware for LC/MS

1. Pre-soak all glassware and equipment as soon after use as is feasible in an ambient temperature 1\% Liquinox solution (10ml/l).
2. Make a fresh warm or hot (between 40°C and 60°C) 1\% solution of Liquinox (10ml/l) for washing when ready to wash.

How do you clean HPLC bottles?

Scrub glassware with detergent in hot tap water using a brush appropriate for the container size. Alconox detergent is recommended, and can be prepared in solution form (~ 0.1-1\%) in tap water in advance. Clean interior and exterior of vessel.

How do you regen a column?

Regeneration Procedure for Normal-Phase Silica Column

1. Disconnect the column and reconnect it to the system, with the flow through the column in the reversed direction.
2. Set the flow at 1 mL/min.
3. Flush column with 50 mL of ethyl acetate.
4. Reconnect the column in the proper flow direction.

How do restaurants clean glassware?

Wiping and Cleaning Glassware, Cutlery and Silverware – Restaurant | BAR | Hotels

1. Prepare hot water.
2. Fill up 25\% of the soaking pan or wine cooler with boiling water.
3. Prepare a dry and cleaned glass cloth.
4. Hold the glass stem / bottom with one end of the cloth over the hot water and let the steam surround the glass.