How do you analyze flow cytometry results?

Flow cytometers utilize properties of fluid dynamics to send cells one at a time through a laser. The optics and computer systems then track the photon emission from excited cells and analyze both the light that scatters past (forward scatter; FSC) and the light that scatters perpendicularly (side scatter; SSC).

What does a flow cytometer analyze?

Flow cytometry is a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations, and analyzing cell size and volume.

What can you learn from flow cytometry?

Flow cytometry assays information Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry. Topics include apoptosis, cell counting, proliferation and viability as well as cell cycle, endocytosis, oxidative stress and more.

How long does it take to get the results of a flow cytometry test?

The typical turn-around time for our diagnostic flow cytometry results is within 1-3 days. Please also note that MRD (Minimal Residual Disease) panels are not available at this time in our flow cytometry laboratory.

How does flow cytometry count cells?

In a flow cytometer, single cells move past the excitation source and the light hitting the cells is either scattered or absorbed and then re-emitted (fluorescence). This scattered or re-emitted light is collected by the detector (see Figure 2).

What is the difference between immunophenotyping and flow cytometry?

The most common use of flow cytometry is in the identification of markers on cells, particularly the immune system or immunophenotyping. It is the desire to detect an increased number of cell subsets of the immune system that has driven the increase in multicolor flow cytometry.

What does Immunophenotypically mean?

Filters. With reference to an immunophenotype. adverb. 1.

Can flow cytometry rule out lymphoma?

Flow cytometry is rapid and appears to be virtually diagnostic of non-Hodgkin’s lymphoma when a majority of cells are B cells with an abnormal kappa/lambda ratio (> 4.0 or < 0.25). Nonhematologic malignancy can be suspected if less than 75 percent of the cells show CD45 (common leukocyte antigen).

What does Leukemia Lymphoma panel by flow mean?

Leukemia and lymphoma analysis by flow cytometry aids in identifying the tumor lineage, which in most cases is identified as T cell, B cell, or myeloid. Lineage identification can provide a confirmatory diagnosis or differential diagnosis, prognosis, and treatment options.

How do you analyze cell cycle data?

The most common method for assessing the cell cycle is to use flow cytometry to measure cellular DNA content. During this process, a fluorescent dye that binds to DNA is incubated with a single cell suspension of permeabilized or fixed cells.

What do the results of my flow cytometry mean?

What Do the Results Mean? Flow cytometry can provide information that is used to diagnose, stage and monitor blood cancers. It can also be used to test for minimal residual disease (MRD), the number of cancer cells remaining in the body after treatment.

What are the advantages of flow cytometry?

The two greatest advantages of flow cytometry are its ability to measure a large number of parameters (2 to 30 or more) on the same sample and its ability to collect information from millions of cells in a matter of seconds.

What does a flow cytometry test show?

It uses a machine that looks for certain substances (markers) on or in cells that help identify what types of cells they are. This test can be used to see if the lymphocytes in a sample of blood contain CLL cells. Flow cytometry can also be used to look for CLL cells in bone marrow or other fluids.

What is the use of flow cytometry?

Introduction to flow cytometry. Flow cytometry is a cell analysis technique that was first used in the 1950s to measure the volume of cells in a rapidly flowing fluid stream