Guidelines

Does RNA polymerase transcribe the operator?

Does RNA polymerase transcribe the operator?

The promoter is the binding site for RNA polymerase, the enzyme that performs transcription. The operator is a negative regulatory site bound by the lac repressor protein. The operator overlaps with the promoter, and when the lac repressor is bound, RNA polymerase cannot bind to the promoter and start transcription.

What part of the operon recruits RNA polymerase?

The promoter sequences of many polymerase II genes are recognized by the TATA-binding protein, which recruits additional transcription factors and RNA polymerase to the promoter.

How would lac operon operates in in E coli?

Answer : When lactose is present , the lac genes are expressed because allolactose binds to the lac repressor protein and keeps it from binding to the lac operator. Small amount of allolactose are formed when lactose enters E. coli.

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What is the function of the lac operator?

What is the function of the lac operator? It produces the lac repressor protein.

Is the operator transcribed?

This allows the RNA polymerase to attach to the DNA segment at the operator sequence. The gene operator will be transcribed, but it will likely be cut out as the mRNA is processed.

How would lac operon operates in in E. coli growing in a culture medium where lactose is present as source of sugar?

In an E. coli cell growing in a growth medium containing glucose as the only carbon source, the lac operon is “off” (not being transcribed). If we add lactose to the growth medium, the lac operon remains “off”, with the cell continuing to utilize glucose.

What is the role of RNA polymerase?

RNA polymerase is an enzyme that is responsible for copying a DNA sequence into an RNA sequence, duyring the process of transcription.

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What role does RNA polymerase play in transcription?

RNA polymerase is the main transcription enzyme. Transcription begins when RNA polymerase binds to a promoter sequence near the beginning of a gene (directly or through helper proteins). RNA polymerase uses one of the DNA strands (the template strand) as a template to make a new, complementary RNA molecule.

What role does RNA polymerase play in transcription mRNA?

RNA polymerase II3 transcribes protein-coding genes into messenger RNA (mRNA). This 12-subunit enzyme works as a complex that directly influences gene expression through its production of pre-mRNA transcripts. These non-coding transcripts can mediate gene expression and the activity of mRNAs after transcription.